BAIT

RTNL1

142893_at, CG18623, CG33113, CG8895, Dmel\CG33113, G9, Retl1, Rtnl-1, Dmel_CG33113
Reticulon-like1
GO Process (2)
GO Function (0)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Drosophila melanogaster
PREY

ATG2

CG1241, Dmel\CG1241, EP3697, Dmel_CG1241
Autophagy-specific gene 2
Drosophila melanogaster

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

In vivo analysis of proteomes and interactomes using Parallel Affinity Capture (iPAC) coupled to mass spectrometry.

Rees JS, Lowe N, Armean IM, Roote J, Johnson G, Drummond E, Spriggs H, Ryder E, Russell S, St Johnston D, Lilley KS

Affinity purification coupled to mass spectrometry provides a reliable method for identifying proteins and their binding partners. In this study we have used Drosophila melanogaster proteins triple tagged with Flag, Strep II, and Yellow fluorescent protein in vivo within affinity pull-down experiments and isolated these proteins in their native complexes from embryos. We describe a pipeline for determining interactomes by ... [more]

Mol. Cell Proteomics Jun. 01, 2011; 10(6);M110.002386 [Pubmed: 21447707]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RTNL1 ATG2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID