BAIT

SEMA-1A

BcDNA:RE36155, CG18405, CT26404, D-Sema 1a, D-Sema I, D-Sema-I, D-SemaI, Dmel\CG18405, Dsema 1a, Dsema-I, Fas-IV, FasIV, Sema 1a, Sema-I, Sema1A, SemaI, l(2)k13702, sema, Dmel_CG18405
CG18405 gene product from transcript CG18405-RE
Drosophila melanogaster
PREY

DSCAM4

CG13672, CG18630, CG33274, CG42330, CG7060, CT21829, Dmel\CG42330, Dmel_CG13672, Dmel_CG33274, Dmel_CG42330
Down syndrome cell adhesion molecule 4
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Drosophila melanogaster

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

In vivo analysis of proteomes and interactomes using Parallel Affinity Capture (iPAC) coupled to mass spectrometry.

Rees JS, Lowe N, Armean IM, Roote J, Johnson G, Drummond E, Spriggs H, Ryder E, Russell S, St Johnston D, Lilley KS

Affinity purification coupled to mass spectrometry provides a reliable method for identifying proteins and their binding partners. In this study we have used Drosophila melanogaster proteins triple tagged with Flag, Strep II, and Yellow fluorescent protein in vivo within affinity pull-down experiments and isolated these proteins in their native complexes from embryos. We describe a pipeline for determining interactomes by ... [more]

Mol. Cell Proteomics Jun. 01, 2011; 10(6);M110.002386 [Pubmed: 21447707]

Throughput

  • High Throughput

Curated By

  • BioGRID