NPL3
Gene Ontology Biological Process
- mRNA export from nucleus [IGI]
- mRNA splicing, via spliceosome [IGI, IMP]
- negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled [IDA, IMP]
- negative regulation of translation [IDA]
- positive regulation of transcription elongation from RNA polymerase II promoter [IDA, IMP]
- translational termination [IGI, IMP]
Gene Ontology Molecular Function
AIR2
Gene Ontology Biological Process
- ncRNA polyadenylation [IDA]
- nuclear mRNA surveillance of mRNA 3'-end processing [IGI]
- nuclear polyadenylation-dependent CUT catabolic process [IGI]
- nuclear polyadenylation-dependent rRNA catabolic process [IGI]
- nuclear polyadenylation-dependent snRNA catabolic process [IGI]
- nuclear polyadenylation-dependent snoRNA catabolic process [IGI]
- nuclear polyadenylation-dependent tRNA catabolic process [IDA]
- tRNA modification [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Novel RING finger proteins, Air1p and Air2p, interact with Hmt1p and inhibit the arginine methylation of Npl3p.
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are involved in the mRNA processing and export and are post-translationally modified by methylation at arginine residues in their arginine-glycine-rich (RGG) domains. We screened the factors that can interact with the RGG domain of Npl3p only in the presence of Hmt1p with the two-hybrid system in Saccharomyces cerevisiae. An isolated clone, YIL079, encodes a novel RING ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AIR2 NPL3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| AIR2 NPL3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| NPL3 AIR2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 271944 | |
| AIR2 NPL3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -3.7917 | BioGRID | 308753 | |
| NPL3 AIR2 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 799185 | |
| NPL3 AIR2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 883178 |
Curated By
- BioGRID