BAIT

AMOTL2

AW549739, C79691, Lccp, MASCOT, mKIAA0989, RP24-221A14.1
angiomotin-like 2
GO Process (1)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

LIN7C

9130007B12Rik, AI303698, AU019331, AW125731, D2Ertd520e, LIN-7-C, LIN-7C, MALS-3, Veli3, RP23-207C2.1
lin-7 homolog C (C. elegans)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The Amot/Patj/Syx signaling complex spatially controls RhoA GTPase activity in migrating endothelial cells.

Ernkvist M, Luna Persson N, Audebert S, Lecine P, Sinha I, Liu M, Schlueter M, Horowitz A, Aase K, Weide T, Borg JP, Majumdar A, Holmgren L

Controlled regulation of Rho GTPase activity is an essential component mediating growth factor-stimulated migration. We have previously shown that angiomotin (Amot), a membrane-associated scaffold protein, plays a critical role during vascular patterning and endothelial migration during embryogenesis. However, the signaling pathways by which Amot controls directional migration are not known. Here we have used peptide pull-down and yeast 2-hybrid (Y2H) ... [more]

Blood Jan. 01, 2009; 113(1);244-53 [Pubmed: 18824598]

Throughput

  • Low Throughput

Additional Notes

  • AmotL2 peptides wer eused in the experiment.

Curated By

  • BioGRID