PREY

MCM3AP

GANP, MAP80, SAC3
minichromosome maintenance complex component 3 associated protein
GO Process (2)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Probing nuclear pore complex architecture with proximity-dependent biotinylation.

Kim DI, Birendra KC, Zhu W, Motamedchaboki K, Doye V, Roux KJ

Proximity-dependent biotin identification (BioID) is a method for identifying protein associations that occur in vivo. By fusing a promiscuous biotin ligase to a protein of interest expressed in living cells, BioID permits the labeling of proximate proteins during a defined labeling period. In this study we used BioID to study the human nuclear pore complex (NPC), one of the largest ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jun. 17, 2014; 111(24);E2453-61 [Pubmed: 24927568]

Throughput

  • High Throughput

Additional Notes

  • BioID

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MCM3AP NUP43
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-4.5683BioGRID
2457853

Curated By

  • BioGRID