BAIT
MED4
ARC36, DRIP36, HSPC126, TRAP36, VDRIP, RP11-90M2.2
mediator complex subunit 4
GO Process (6)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
KIF3A
FLA10, KLP-20
kinesin family member 3A
GO Process (11)
GO Function (5)
GO Component (7)
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- blood coagulation [TAS]
- cilium assembly [ISS]
- cilium morphogenesis [IBA]
- membrane organization [TAS]
- metabolic process [IBA, TAS]
- microtubule-based movement [IBA, TAS]
- neurogenesis [IBA]
- organelle organization [TAS]
- plus-end-directed vesicle transport along microtubule [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes.
Mapping protein-protein interactions for chromatin-associated proteins remains challenging. Here we explore the use of BioID, a proximity biotinylation approach in which a mutated biotin ligase (BirA*) is fused to a bait of interest, allowing for the local activation of biotin and subsequent biotinylation of proteins in the bait vicinity. BioID allowed for successful interactome mapping of core histones and members ... [more]
J Proteomics Apr. 06, 2015; 118(0);81-94 [Pubmed: 25281560]
Throughput
- High Throughput
Additional Notes
- BioID
Curated By
- BioGRID