BAIT
MED4
ARC36, DRIP36, HSPC126, TRAP36, VDRIP, RP11-90M2.2
mediator complex subunit 4
GO Process (6)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TLN1
ILWEQ, TLN, RP11-112J3.1
talin 1
GO Process (12)
GO Function (5)
GO Component (9)
Gene Ontology Biological Process
- activation of signaling protein activity involved in unfolded protein response [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell-cell junction assembly [TAS]
- cellular component movement [NAS]
- cellular protein metabolic process [TAS]
- cytoskeletal anchoring at plasma membrane [NAS]
- endoplasmic reticulum unfolded protein response [TAS]
- muscle contraction [TAS]
- platelet activation [TAS]
- platelet aggregation [IMP]
- platelet degranulation [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes.
Mapping protein-protein interactions for chromatin-associated proteins remains challenging. Here we explore the use of BioID, a proximity biotinylation approach in which a mutated biotin ligase (BirA*) is fused to a bait of interest, allowing for the local activation of biotin and subsequent biotinylation of proteins in the bait vicinity. BioID allowed for successful interactome mapping of core histones and members ... [more]
J Proteomics Apr. 06, 2015; 118(0);81-94 [Pubmed: 25281560]
Throughput
- High Throughput
Additional Notes
- BioID
Curated By
- BioGRID