BAIT

MED4

ARC36, DRIP36, HSPC126, TRAP36, VDRIP, RP11-90M2.2
mediator complex subunit 4
Homo sapiens
PREY

DST

BP240, BPA, BPAG1, CATX-15, CATX15, D6S1101, DMH, DT, EBSB2, HSAN6, MACF2, RP3-361I14.2
dystonin
Glioblastoma Project
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes.

Lambert JP, Tucholska M, Go C, Knight JD, Gingras AC

Mapping protein-protein interactions for chromatin-associated proteins remains challenging. Here we explore the use of BioID, a proximity biotinylation approach in which a mutated biotin ligase (BirA*) is fused to a bait of interest, allowing for the local activation of biotin and subsequent biotinylation of proteins in the bait vicinity. BioID allowed for successful interactome mapping of core histones and members ... [more]

J Proteomics Apr. 06, 2015; 118(0);81-94 [Pubmed: 25281560]

Throughput

  • High Throughput

Additional Notes

  • BioID

Curated By

  • BioGRID