An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Proteomic Analysis of the EWS-Fli-1 Interactome Reveals the Role of the Lysosome in EWS-Fli-1 Turnover.

Elzi DJ, Song M, Hakala K, Weintraub ST, Shiio Y

Ewing sarcoma is a cancer of bone and soft tissue in children that is characterized by a chromosomal translocation involving EWS and an Ets family transcription factor, most commonly Fli-1. EWS-Fli-1 fusion accounts for 85% of cases. The growth and survival of Ewing sarcoma cells are critically dependent on EWS-Fli-1. A large body of evidence has established that EWS-Fli-1 functions ... [more]

J. Proteome Res. Aug. 01, 2014; 13(8);3783-91 [Pubmed: 24999758]

Throughput

High Throughput

Additional Notes

BioID

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
EWSR1 VPS72	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Rual JF (2005)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

EWSR1

Gene Ontology Molecular Function

RNA binding [TAS]identical protein binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

VPS72

Gene Ontology Biological Process