BAIT

DSK1

SPBC530.14c
SR protein-specific kinase Dsk1
Schizosaccharomyces pombe (972h)
PREY

RPS101

rps1-1, rps3a-1, SPAC13G6.02c
40S ribosomal protein S3a
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Biochemical Activity (Phosphorylation)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

SR protein kinases promote splicing of nonconsensus introns.

Lipp JJ, Marvin MC, Shokat KM, Guthrie C

Phosphorylation of the spliceosome is essential for RNA splicing, yet how and to what extent kinase signaling affects splicing have not been defined on a genome-wide basis. Using a chemical genetic approach, we show in Schizosaccharomyces pombe that the SR protein kinase Dsk1 is required for efficient splicing of introns with suboptimal splice sites. Systematic substrate mapping in fission yeast ... [more]

Nat. Struct. Mol. Biol. Aug. 01, 2015; 22(8);611-7 [Pubmed: 26167880]

Throughput

  • High Throughput

Additional Notes

  • We labeled fission yeast extracts with N6-phenethyl-ATP-gammaS in the presence of either recombinant WT or analog-sensitive Dsk1. We then purified thiophosphorylated peptides and identified 131 direct Dsk1 substrates as well as their phosphorylation sites by MS

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DSK1 RPS101
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.1164BioGRID
770267

Curated By

  • BioGRID