Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Arabidopsis GERANYLGERANYL DIPHOSPHATE SYNTHASE 11 is a hub isozyme required for the production of most photosynthesis-related isoprenoids.

Ruiz-Sola M A, Coman D, Beck G, Barja MV, Colinas M, Graf A, Welsch R, Ruetimann P, Buehlmann P, Bigler L, Gruissem W, Rodriguez-Concepcion M, Vranova E

Most plastid isoprenoids, including photosynthesis-related metabolites such as carotenoids and the side chain of chlorophylls, tocopherols (vitamin E), phylloquinones (vitamin K), and plastoquinones, derive from geranylgeranyl diphosphate (GGPP) synthesized by GGPP synthase (GGPPS) enzymes. Seven out of 10 functional GGPPS isozymes in Arabidopsis thaliana reside in plastids. We aimed to address the function of different GGPPS paralogues for plastid isoprenoid ... [more]

New Phytol. Jan. 01, 2016; 209(1);252-64 [Pubmed: 26224411]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GGPS1 SPS1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Ruiz-Sola M A (2016)	Low	-	BioGRID	-
GGPS1 SPS1	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Ruiz-Sola M A (2016)	Low	-	BioGRID	1429255

Curated By

BioGRID

Interaction Summary

GGPS1

Gene Ontology Biological Process

Gene Ontology Molecular Function

dimethylallyltranstransferase activity [IDA]farnesyltranstransferase activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

SPS1