An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

SR protein kinases promote splicing of nonconsensus introns.

Lipp JJ, Marvin MC, Shokat KM, Guthrie C

Phosphorylation of the spliceosome is essential for RNA splicing, yet how and to what extent kinase signaling affects splicing have not been defined on a genome-wide basis. Using a chemical genetic approach, we show in Schizosaccharomyces pombe that the SR protein kinase Dsk1 is required for efficient splicing of introns with suboptimal splice sites. Systematic substrate mapping in fission yeast ... [more]

Nat. Struct. Mol. Biol. Aug. 01, 2015; 22(8);611-7 [Pubmed: 26167880]

Throughput

High Throughput

Additional Notes

We labeled HeLa extracts with N6-phenethyl-ATP-gammaS in the presence of either recombinant WT or analog-sensitive Srpk2. We then purified thiophosphorylated peptides and identified Srpk2 substrates as well as their phosphorylation sites by MS

Curated By

BioGRID

Interaction Summary

SRPK2

Gene Ontology Biological Process

Gene Ontology Molecular Function

14-3-3 protein binding [ISS]ATP binding [IDA]magnesium ion binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

AP1M1