BAIT

CEP97

2810403B08Rik, LRRIQ2
centrosomal protein 97kDa
GO Process (0)
GO Function (1)
GO Component (4)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

CLTC

CHC, CHC17, CLH-17, CLTCL2, Hc
clathrin, heavy chain (Hc)
Autophagy Project
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.

Gupta GD, Coyaud E, Goncalves J, Mojarad BA, Liu Y, Wu Q, Gheiratmand L, Comartin D, Tkach JM, Cheung SW, Bashkurov M, Hasegan M, Knight JD, Lin ZY, Schueler M, Hildebrandt F, Moffat J, Gingras AC, Raught B, Pelletier L

The centrosome is the primary microtubule organizing center of the cells and templates the formation of cilia, thereby operating at a nexus of critical cellular functions. Here, we use proximity-dependent biotinylation (BioID) to map the centrosome-cilium interface; with 58 bait proteins we generate a protein topology network comprising >7,000 interactions. Analysis of interaction profiles coupled with high resolution phenotypic profiling implicates a ... [more]

Cell Dec. 03, 2015; 163(6);1484-99 [Pubmed: 26638075]

Throughput

  • High Throughput

Additional Notes

  • BioID

Curated By

  • BioGRID