An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.

Gupta GD, Coyaud E, Goncalves J, Mojarad BA, Liu Y, Wu Q, Gheiratmand L, Comartin D, Tkach JM, Cheung SW, Bashkurov M, Hasegan M, Knight JD, Lin ZY, Schueler M, Hildebrandt F, Moffat J, Gingras AC, Raught B, Pelletier L

The centrosome is the primary microtubule organizing center of the cells and templates the formation of cilia, thereby operating at a nexus of criticalÂ cellular functions. Here, we use proximity-dependent biotinylation (BioID) to map the centrosome-cilium interface; with 58 bait proteins we generate a protein topology network comprising >7,000 interactions. Analysis of interaction profiles coupled withÂ high resolution phenotypic profiling implicates a ... [more]

Cell Dec. 03, 2015; 163(6);1484-99 [Pubmed: 26638075]

Throughput

High Throughput

Additional Notes

BioID

Curated By

BioGRID

Interaction Summary

SCLT1

Gene Ontology Biological Process

Gene Ontology Cellular Component

PRKAR2A

Gene Ontology Biological Process

Gene Ontology Molecular Function

cAMP-dependent protein kinase inhibitor activity [IDA]cAMP-dependent protein kinase regulator activity [IDA, NAS]protein binding [IPI]protein kinase A catalytic subunit binding [IPI]ubiquitin protein ligase binding [IDA]