BAIT

HPR1

TRF1, L000000808, YDR138W
Subunit of THO/TREX complexes; this complex couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p
Saccharomyces cerevisiae (S288c)
PREY

TOP1

MAK1, MAK17, DNA topoisomerase 1, L000002319, YOL006C
Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Topological constraints impair RNA polymerase II transcription and causes instability of plasmid-borne convergent genes.

Garcia-Rubio ML, Aguilera A

Despite the theoretical bases for the association of topoisomerases and supercoiling changes with transcription and replication, our knowledge of the impact of topological constraints on transcription and replication is incomplete. Although mutation of topoisomerases affects expression and stability of the rDNA region it is not clear whether the same is the case for RNAPII transcription and genome integrity in other ... [more]

Nucleic Acids Res. Feb. 01, 2012; 40(3);1050-64 [Pubmed: 21998294]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: mitotic recombination (APO:0000225)

Additional Notes

  • Figure S3
  • genetic complex
  • hpr1 top1 top2-1 triple mutant has increased recombination frequency

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HPR1 TOP1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
157166
HPR1 TOP1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
160821
TOP1 HPR1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
160714
TOP1 HPR1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
159022

Curated By

  • BioGRID