BAIT

BDF2

L000004431, YDL070W
Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress; BDF2 has a paralog, BDF1, that arose from the whole genome duplication
GO Process (1)
GO Function (4)
GO Component (1)
Saccharomyces cerevisiae (S288c)
PREY

ASF1

CIA1, nucleosome assembly factor ASF1, L000000126, YJL115W
Nucleosome assembly factor; involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition; relocalizes to the cytosol in response to hypoxia
Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Identification and characterization of CIA/ASF1 as an interactor of bromodomains associated with TFIID.

Chimura T, Kuzuhara T, Horikoshi M

General transcription initiation factor IID (TFIID) plays a central and critical role in transcription initiation from both naked and chromatin templates. Although interaction between several DNA-binding proteins and TFIID were identified and well characterized, functional linkage between TFIID and chromatin factors has remained to be elucidated. Here we show the identification and characterization of human CIA/hASF1 (identified previously as a ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jul. 09, 2002; 99(14);9334-9 [Pubmed: 12093919]

Throughput

  • Low Throughput

Curated By

  • BioGRID