An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning.

Kirli K, Karaca S, Dehne HJ, Samwer M, Pan KT, Lenz C, Urlaub H, Goerlich D

CRM1 is a highly conserved, RanGTPase-driven exportin that caries proteins and RNPs from the nucleus to the cytoplasm. We now explored the cargo-spectrum of CRM1 in depth and identified surprisingly large numbers, namely >700 export substrates from the yeast S. cerevisiae, â‰ˆ 1000 from Xenopus oocytes and >1050 from human cells. In addition, we quantified the partitioning of â‰ˆ5000 unique ... [more]

Elife Dec. 17, 2015; 4(0); [Pubmed: 26673895]

Throughput

High Throughput

Additional Notes

Cargo A
Intreaction in the presence of RanGTP

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
EIF3C XPO1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Horlbeck MA (2018)	High	-5.4843	BioGRID	2457021

Curated By

BioGRID

Interaction Summary

XPO1

Gene Ontology Biological Process

Gene Ontology Molecular Function

nucleocytoplasmic transporter activity [IMP]protein binding [IPI]transporter activity [TAS]

Gene Ontology Cellular Component

EIF3C

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]translation initiation factor activity [IC, IDA]