PREY

NFKB1

EBP-1, KBF1, NF-kB1, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, NFkappaB, p105, p50
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
GO Process (40)
GO Function (9)
GO Component (6)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning.

Kirli K, Karaca S, Dehne HJ, Samwer M, Pan KT, Lenz C, Urlaub H, Goerlich D

CRM1 is a highly conserved, RanGTPase-driven exportin that caries proteins and RNPs from the nucleus to the cytoplasm. We now explored the cargo-spectrum of CRM1 in depth and identified surprisingly large numbers, namely >700 export substrates from the yeast S. cerevisiae, ≈ 1000 from Xenopus oocytes and >1050 from human cells. In addition, we quantified the partitioning of ≈5000 unique ... [more]

Elife Dec. 17, 2015; 4(0); [Pubmed: 26673895]

Throughput

  • High Throughput

Additional Notes

  • Cargo B
  • Intreaction in the presence of RanGTP

Curated By

  • BioGRID