PREY

COIL

CLN80, p80-coilin
coilin
GO Process (0)
GO Function (3)
GO Component (6)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning.

Kirli K, Karaca S, Dehne HJ, Samwer M, Pan KT, Lenz C, Urlaub H, Goerlich D

CRM1 is a highly conserved, RanGTPase-driven exportin that caries proteins and RNPs from the nucleus to the cytoplasm. We now explored the cargo-spectrum of CRM1 in depth and identified surprisingly large numbers, namely >700 export substrates from the yeast S. cerevisiae, ≈ 1000 from Xenopus oocytes and >1050 from human cells. In addition, we quantified the partitioning of ≈5000 unique ... [more]

Elife Dec. 17, 2015; 4(0); [Pubmed: 26673895]

Throughput

  • High Throughput

Additional Notes

  • Intreaction in the presence of RanGTP
  • Low Abundant Cargo

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
COIL XPO1
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High6.28BioGRID
2983837

Curated By

  • BioGRID