BAIT

CRM1

KAP124, XPO1, exportin CRM1, L000000420, YGR218W
Major karyopherin; involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin
Saccharomyces cerevisiae (S288c)
PREY

AIR2

YDL175C
RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication; Air2p and Air1p have nonredundant roles in regulation of substrate specificity of the exosome
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning.

Kirli K, Karaca S, Dehne HJ, Samwer M, Pan KT, Lenz C, Urlaub H, Goerlich D

CRM1 is a highly conserved, RanGTPase-driven exportin that caries proteins and RNPs from the nucleus to the cytoplasm. We now explored the cargo-spectrum of CRM1 in depth and identified surprisingly large numbers, namely >700 export substrates from the yeast S. cerevisiae, ≈ 1000 from Xenopus oocytes and >1050 from human cells. In addition, we quantified the partitioning of ≈5000 unique ... [more]

Elife Dec. 17, 2015; 4(0); [Pubmed: 26673895]

Throughput

  • High Throughput

Additional Notes

  • Intreaction in the presence of RanGTP
  • Low Abundant Cargo

Curated By

  • BioGRID