Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A RNA polymerase III-based two-hybrid system to study RNA polymerase II transcriptional regulators.

Marsolier MC, Prioleau MN, Sentenac A

In a previous study, we explored the mechanisms of SNR6 gene activation by grafting a heterologous DNA-binding domain, GAL4-(1-147), to various components of the yeast RNA polymerase III transcription system. Here, we demonstrate that a modified SNR6 gene harboring GAL4-binding sites (UAS(G)-SNR6) can be efficiently activated via an intervening, unrelated protein-protein interaction, thus laying the foundations of a RNA polymerase ... [more]

J. Mol. Biol. May. 02, 1997; 268(2);243-9 [Pubmed: 9159467]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PRP21 PRP11	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
PRP11 PRP21	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
PRP21 PRP11	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Legrain P (1993)	Low	-	BioGRID	-
PRP11 PRP21	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3602650
PRP11 PRP21	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Zhang Z (2021)	High	-	BioGRID	-
PRP21 PRP11	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Zhang Z (2021)	High	-	BioGRID	-
PRP11 PRP21	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.4585	BioGRID	1923099
PRP21 PRP11	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.519	BioGRID	1939626
PRP11 PRP21	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Wells SE (1994)	Low	-	BioGRID	-
PRP11 PRP21	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Wiest DK (1996)	Low	-	BioGRID	-
PRP11 PRP21	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fromont-Racine M (1997)	High	-	BioGRID	-
PRP21 PRP11	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fromont-Racine M (1997)	High	-	BioGRID	-
PRP21 PRP11	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Rain JC (1996)	Low	-	BioGRID	-
PRP11 PRP21	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Uetz P (2000)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PRP21

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [IDA]

Gene Ontology Cellular Component

PRP11