An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex.

Guarani V, Paulo J, Zhai B, Huttlin EL, Gygi SP, Harper JW

Complex I (CI) of the electron transport chain, a large membrane-embedded NADH dehydrogenase, couples electron transfer to the release of protons into the mitochondrial inner membrane space to promote ATP production through ATP synthase. In addition to being a central conduit for ATP production, CI activity has been linked to neurodegenerative disorders, including Parkinson's disease. CI is built in a ... [more]

Mol. Cell. Biol. Mar. 01, 2014; 34(5);847-61 [Pubmed: 24344204]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TIMMDC1 NDUFA9	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Guarani V (2014)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

NDUFA9

Gene Ontology Biological Process

Gene Ontology Molecular Function

NADH dehydrogenase (ubiquinone) activity [NAS]NADH dehydrogenase activity [IMP, NAS]protein binding [IPI]protein complex binding [IDA]

Gene Ontology Cellular Component

TIMMDC1