BAIT
IQGAP1
HUMORFA01, SAR1, p195
IQ motif containing GTPase activating protein 1
GO Process (13)
GO Function (9)
GO Component (17)
Gene Ontology Biological Process
- cellular response to calcium ion [IDA]
- cellular response to epidermal growth factor stimulus [IMP]
- energy reserve metabolic process [TAS]
- epidermal growth factor receptor signaling pathway [IMP]
- glomerular visceral epithelial cell development [ISS]
- negative regulation of catalytic activity [TAS]
- neuron projection extension [IMP]
- positive regulation of GTPase activity [TAS]
- positive regulation of protein kinase activity [IMP]
- positive regulation of protein serine/threonine kinase activity [IDA]
- regulation of insulin secretion [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- GTPase activator activity [TAS]
- GTPase inhibitor activity [TAS]
- calcium ion binding [TAS]
- calmodulin binding [IPI]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine kinase activator activity [IDA]
- GTPase activator activity [TAS]
- GTPase inhibitor activity [TAS]
- calcium ion binding [TAS]
- calmodulin binding [IPI]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine kinase activator activity [IDA]
Gene Ontology Cellular Component
- actin cytoskeleton [ISS]
- actin filament [TAS]
- axon [ISS]
- cell junction [IDA]
- cytoplasm [IDA]
- cytoplasmic ribonucleoprotein granule [IDA]
- extracellular vesicular exosome [IDA]
- extrinsic component of cytoplasmic side of plasma membrane [IDA]
- focal adhesion [IDA]
- growth cone [ISS]
- microtubule [IDA]
- microtubule cytoskeleton [ISS]
- midbody [IDA]
- neuron projection [ISS]
- nucleoplasm [IDA]
- plasma membrane [IDA, TAS]
- slit diaphragm [ISS]
Homo sapiens
PREY
PPP1R12A
M130, MBS, MYPT1
protein phosphatase 1, regulatory subunit 12A
GO Process (10)
GO Function (6)
GO Component (8)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- centrosome organization [IMP]
- mitotic cell cycle [TAS]
- mitotic nuclear division [IMP]
- negative regulation of catalytic activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein dephosphorylation [IMP]
- regulation of cell adhesion [IDA]
- regulation of myosin-light-chain-phosphatase activity [IDA]
- signal transduction [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances.
The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have generated a library of HeLa cell lines expressing 1,125 GFP-tagged proteins ... [more]
Cell Oct. 22, 2015; 163(3);712-23 [Pubmed: 26496610]
Throughput
- High Throughput
Additional Notes
- interaction detected by quantitative BAC-GFP interactomics (QUBIC)
Curated By
- BioGRID