An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances.

Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M

The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have generated a library of HeLa cell lines expressing 1,125 GFP-tagged proteins ... [more]

Cell Oct. 22, 2015; 163(3);712-23 [Pubmed: 26496610]

Throughput

High Throughput

Additional Notes

interaction detected by quantitative BAC-GFP interactomics (QUBIC)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SOD1 CCS	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	0.9998	BioGRID	2228590
SOD1 CCS	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9999	BioGRID	3108734
CCS SOD1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Marcon E (2014)	High	35.2717	BioGRID	2943696
SOD1 CCS	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Brady GF (2010)	Low	-	BioGRID	-
CCS SOD1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Casareno RL (1998)	Low	-	BioGRID	-
CCS SOD1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Huppke P (2012)	Low	-	BioGRID	-
SOD1 CCS	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Sala FA (2019)	Low	-	BioGRID	-
SOD1 CCS	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Wan C (2015)	High	0.1478	BioGRID	1259720
CCS SOD1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Casareno RL (1998)	Low	-	BioGRID	-
CCS SOD1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Tao TY (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SOD1

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rac GTPase binding [IDA]chaperone binding [IPI]copper ion binding [IDA]identical protein binding [IPI]protein binding [IPI]protein homodimerization activity [NAS]protein phosphatase 2B binding [IDA]superoxide dismutase activity [EXP, IDA, TAS]zinc ion binding [IDA]

Gene Ontology Cellular Component

CCS

Gene Ontology Biological Process

Gene Ontology Molecular Function

copper ion binding [IBA]copper ion transmembrane transporter activity [TAS]protein binding [IPI]protein disulfide oxidoreductase activity [TAS]superoxide dismutase activity [IBA]zinc ion binding [IBA]