ARG80
Gene Ontology Biological Process
Gene Ontology Molecular Function
MCM1
Gene Ontology Biological Process
- DNA replication initiation [IMP]
- negative regulation of arginine catabolic process by negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of mating-type specific transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of arginine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of mating-type specific transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription involved in G2/M transition of mitotic cell cycle [IMP]
- regulation of mating type switching [IMP]
Gene Ontology Molecular Function- DNA replication origin binding [IDA]
- RNA polymerase II activating transcription factor binding [IMP, IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding, bending [IDA, IMP]
- RNA polymerase II repressing transcription factor binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IDA, IPI]
- DNA replication origin binding [IDA]
- RNA polymerase II activating transcription factor binding [IMP, IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding, bending [IDA, IMP]
- RNA polymerase II repressing transcription factor binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IDA, IPI]
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Swapping functional specificity of a MADS box protein: residues required for Arg80 regulation of arginine metabolism.
Arg80 and Mcm1, two members of the MADS box family of DNA-binding proteins, regulate the metabolism of arginine in association with Arg81, the arginine sensor. In spite of the high degree of sequence conservation between the MADS box domains of the Arg80 and Mcm1 proteins (56 of 81 amino acids), these domains are not interchangeable. To determine which amino acids ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ARG80 MCM1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ARG80 MCM1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| MCM1 ARG80 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID