BAIT
CENPE
312kDa, AU019344, BC049989, C530022J18, CENP-E, Kif10
centromere protein E
GO Process (15)
GO Function (4)
GO Component (15)
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- attachment of mitotic spindle microtubules to kinetochore [ISO]
- attachment of spindle microtubules to kinetochore [IMP, TAS]
- establishment of protein localization [IMP]
- metabolic process [ISO]
- microtubule-based movement [IBA]
- mitotic metaphase plate congression [ISO]
- mitotic spindle assembly checkpoint [IMP]
- positive regulation of attachment of spindle microtubules to kinetochore [IMP]
- positive regulation of chromosome segregation [IMP]
- positive regulation of mitotic metaphase/anaphase transition [IMP]
- positive regulation of protein kinase activity [IDA, ISO]
- regulation of developmental process [IMP]
- regulation of mitosis [IMP]
- regulation of mitotic metaphase/anaphase transition [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell [IMP]
- chromosome [ISO]
- chromosome, centromeric region [ISO]
- condensed chromosome outer kinetochore [IDA]
- condensed chromosome, centromeric region [ISO]
- condensed nuclear chromosome kinetochore [IDA]
- cytoplasm [ISO]
- kinesin complex [IBA]
- kinetochore [IDA, ISO]
- membrane [ISO]
- microtubule [ISO]
- microtubule cytoskeleton [ISO]
- midbody [ISO]
- mitotic spindle midzone [ISO]
- nucleus [ISO]
Mus musculus
PREY
DCTN1
DAP-150, DP-150, P135
dynactin 1
GO Process (9)
GO Function (1)
GO Component (8)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- cellular protein metabolic process [TAS]
- endoplasmic reticulum unfolded protein response [TAS]
- mitotic cell cycle [TAS]
- mitotic nuclear division [NAS]
- nervous system development [NAS]
- retrograde transport, endosome to Golgi [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances.
The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have generated a library of HeLa cell lines expressing 1,125 GFP-tagged proteins ... [more]
Cell Oct. 22, 2015; 163(3);712-23 [Pubmed: 26496610]
Throughput
- High Throughput
Additional Notes
- interaction detected by quantitative BAC-GFP interactomics (QUBIC)
Curated By
- BioGRID