BAIT
ACF4
YJR083C
Protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate
GO Process (0)
GO Function (0)
GO Component (0)
Saccharomyces cerevisiae (S288c)
PREY
ACF4
YJR083C
Protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate
GO Process (0)
GO Function (0)
GO Component (0)
Saccharomyces cerevisiae (S288c)
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A computationally directed screen identifying interacting coiled coils from Saccharomyces cerevisiae.
Computational methods can frequently identify protein-interaction motifs in otherwise uncharacterized open reading frames. However, the identification of candidate ligands for these motifs (e.g., so that partnering can be determined experimentally in a directed manner) is often beyond the scope of current computational capabilities. One exception is provided by the coiled-coil interaction motif, which consists of two or more alpha helices ... [more]
Proc. Natl. Acad. Sci. U.S.A. Nov. 21, 2000; 97(24);13203-8 [Pubmed: 11087867]
Throughput
- High Throughput
Curated By
- BioGRID