BEM1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ACT1
Gene Ontology Biological Process
- DNA repair [IDA]
- actomyosin contractile ring contraction [IDA, IMP]
- ascospore wall assembly [IDA]
- budding cell isotropic bud growth [TAS]
- cellular response to oxidative stress [IGI]
- chronological cell aging [IMP]
- endocytosis [IMP]
- establishment of cell polarity [IGI]
- establishment of mitotic spindle orientation [TAS]
- exocytosis [TAS]
- fungal-type cell wall organization [TAS]
- histone acetylation [IDA]
- mitochondrion inheritance [TAS]
- protein secretion [IGI, IMP]
- vacuole inheritance [IGI, IMP]
- vesicle transport along actin filament [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Pheromone response in yeast: association of Bem1p with proteins of the MAP kinase cascade and actin.
Haploid cells of the yeast Saccharomyces cerevisiae respond to mating pheromones with polarized growth toward the mating partner. This morphological response requires the function of the cell polarity establishment protein Bem1p. Immunochemical and two-hybrid protein interaction assays revealed that Bem1p interacts with two components of the pheromone-responsive mitogen-activated protein (MAP) kinase cascade, Ste20p and Ste5p, as well as with actin. ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BEM1 ACT1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | 204490 | |
| ACT1 BEM1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1496 | BioGRID | 376943 | |
| ACT1 BEM1 | Synthetic Haploinsufficiency Synthetic Haploinsufficiency A genetic interaction is inferred when mutations or deletions in separate genes, at least one of which is hemizygous, cause a minimal phenotype alone but result in lethality when combined in the same cell under a given condition. | High | - | BioGRID | 281633 |
Curated By
- BioGRID