BAIT

RPO21

RPB1, RPB220, SUA8, DNA-directed RNA polymerase II core subunit RPO21, B220, L000001744, YDL140C

RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime

GO Process (2)

GO Function (2)

GO Component (3)

Gene Ontology Biological Process

transcription from RNA polymerase II promoter [IMP]

translesion synthesis [IMP]

Gene Ontology Molecular Function

RNA polymerase II activity [IDA]
RNA-directed RNA polymerase activity [IDA]

Gene Ontology Cellular Component

DNA-directed RNA polymerase II, core complex [IDA]

mitochondrion [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RPB10

DNA-directed RNA polymerase core subunit RPB10, ABC10-beta, L000001684, YOR210W

RNA polymerase subunit ABC10-beta; common to RNA polymerases I, II, and III

GO Process (4)

GO Function (5)

GO Component (3)

Gene Ontology Biological Process

tRNA transcription from RNA polymerase III promoter [IDA]

transcription from RNA polymerase I promoter [IDA, IGI, IMP]

transcription from RNA polymerase II promoter [IMP]

transcription from RNA polymerase III promoter [IC]

Gene Ontology Molecular Function

RNA polymerase I activity [IDA]
RNA polymerase II activity [IDA]
RNA polymerase III activity [IDA]
RNA-directed RNA polymerase activity [IDA]
zinc ion binding [IDA]

Gene Ontology Cellular Component

DNA-directed RNA polymerase I complex [IDA]

DNA-directed RNA polymerase II, core complex [IDA]

DNA-directed RNA polymerase III complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A protein-protein interaction map of yeast RNA polymerase III.

Flores A, Briand JF, Gadal O, Andrau JC, Rubbi L, Van Mullem V, Boschiero C, Goussot M, Marck C, Carles C, Thuriaux P, Sentenac A, Werner M

The structure of the yeast RNA polymerase (pol) III was investigated by exhaustive two-hybrid screening using a library of random genomic fragments fused to the Gal4 activation domain. This procedure allowed us to identify contacts between individual polypeptides, localize the contact domains, and deduce a protein-protein interaction map of the multisubunit enzyme. In all but one case, pol III subunits ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jul. 06, 1999; 96(14);7815-20 [Pubmed: 10393904]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPO21 RPB10	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Eyboulet F (2020)	High	-	BioGRID	-
RPO21 RPB10	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
RPO21 RPB10	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	McCann TS (2016)	Low	-	BioGRID	2202807
RPO21 RPB10	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	6	BioGRID	3605269
RPO21 RPB10	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Roether S (2010)	Low	-	BioGRID	-
RPO21 RPB10	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Kettenberger H (2004)	Low	-	BioGRID	-
RPB10 RPO21	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.4044	BioGRID	1952975

Curated By

BioGRID

Interaction Summary

RPO21

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II activity [IDA]RNA-directed RNA polymerase activity [IDA]

Gene Ontology Cellular Component

RPB10

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase I activity [IDA]RNA polymerase II activity [IDA]RNA polymerase III activity [IDA]RNA-directed RNA polymerase activity [IDA]zinc ion binding [IDA]

Gene Ontology Cellular Component

Two-hybrid

Publication

A protein-protein interaction map of yeast RNA polymerase III.

Throughput

Related interactions

Curated By

RNA polymerase II activity [IDA]
RNA-directed RNA polymerase activity [IDA]

RNA polymerase I activity [IDA]
RNA polymerase II activity [IDA]
RNA polymerase III activity [IDA]
RNA-directed RNA polymerase activity [IDA]
zinc ion binding [IDA]