CESA4
Gene Ontology Biological Process
- cell wall thickening [IMP]
- cellulose biosynthetic process [IMP, TAS]
- defense response to bacterium [IMP]
- defense response to fungus [IMP]
- ethylene-activated signaling pathway [IGI]
- jasmonic acid mediated signaling pathway [IGI]
- plant-type cell wall biogenesis [TAS]
- plant-type secondary cell wall biogenesis [IMP]
- salicylic acid mediated signaling pathway [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
IRX1
Gene Ontology Biological Process
- cell wall thickening [IMP]
- cellulose biosynthetic process [IMP]
- defense response to bacterium [IMP]
- defense response to fungus [IMP]
- ethylene-activated signaling pathway [IGI]
- jasmonic acid mediated signaling pathway [IGI]
- plant-type secondary cell wall biogenesis [IMP]
- positive regulation of abscisic acid biosynthetic process [IGI]
- response to osmotic stress [IMP]
- response to water deprivation [IMP]
- salicylic acid mediated signaling pathway [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Interactions between membrane-bound cellulose synthases involved in the synthesis of the secondary cell wall.
It has not yet been reported how the secondary CESA (cellulose synthase) proteins are organized in the rosette structure. A membrane-based yeast two-hybrid (MbYTH) approach was used to analyze the interactions between the CESA proteins involved in secondary cell wall synthesis of Arabidopsis and the findings were confirmed in planta by bimolecular fluorescence complementation (BiFC) assay. Results indicated that although ... [more]
Throughput
- Low Throughput
Additional Notes
- BiFC assay
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| IRX1 CESA4 | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | 1504085 | |
| CESA4 IRX1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| IRX1 CESA4 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| CESA4 IRX1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| IRX1 CESA4 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID