A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

DNA repair and global sumoylation are regulated by distinct Ubc9 noncovalent complexes.

Prudden J, Perry JJ, Nie M, Vashisht AA, Arvai AS, Hitomi C, Guenther G, Wohlschlegel JA, Tainer JA, Boddy MN

Global sumoylation, SUMO chain formation, and genome stabilization are all outputs generated by a limited repertoire of enzymes. Mechanisms driving selectivity for each of these processes are largely uncharacterized. Here, through crystallographic analyses we show that the SUMO E2 Ubc9 forms a noncovalent complex with a SUMO-like domain of Rad60 (SLD2). Ubc9:SLD2 and Ubc9:SUMO noncovalent complexes are structurally analogous, suggesting ... [more]

Mol. Cell. Biol. Jun. 01, 2011; 31(11);2299-310 [Pubmed: 21444718]

Throughput

Low Throughput

Ontology Terms

phenotype: viability (APO:0000111)

Additional Notes

Interactor A: null
Interactor B: SUMOD81R

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAD51 PMT3	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Ryan CJ (2012)	High	-4.7626	BioGRID	775269

Curated By

BioGRID

Interaction Summary

RAD51

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]ATPase activity [IDA]double-stranded DNA binding [IDA]double-stranded telomeric DNA binding [IDA]protein binding [IPI]protein-DNA loading ATPase activity [TAS]recombinase activity [IDA]single-stranded DNA binding [IDA]single-stranded telomeric DNA binding [IDA]

Gene Ontology Cellular Component

PMT3