BAIT

BRR2

PRP44, RSS1, SLT22, SNU246, L000003100, L000003283, YER172C
RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

PBP1

MRS16, L000003967, YGR178C
Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
GO Process (4)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Brr2p carboxy-terminal Sec63 domain modulates Prp16 splicing RNA helicase.

Cordin O, Hahn D, Alexander R, Gautam A, Saveanu C, Barrass JD, Beggs JD

RNA helicases are essential for virtually all cellular processes, however, their regulation is poorly understood. The activities of eight RNA helicases are required for pre-mRNA splicing. Amongst these, Brr2p is unusual in having two helicase modules, of which only the amino-terminal helicase domain appears to be catalytically active. Using genetic and biochemical approaches, we investigated interaction of the carboxy-terminal helicase ... [more]

Nucleic Acids Res. Dec. 16, 2014; 42(22);13897-910 [Pubmed: 25428373]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • Genetic Interaction Mapping screen
  • Table S4
  • brr2L1930P, brr2L1951P alleles used

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BRR2 PBP1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2217BioGRID
1977992

Curated By

  • BioGRID