BAIT

MAPK8

JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c
mitogen-activated protein kinase 8
GO Process (36)
GO Function (5)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

RHOA

ARH12, ARHA, RHO12, RHOH12
ras homolog family member A
GO Process (29)
GO Function (3)
GO Component (8)

Gene Ontology Molecular Function

Homo sapiens

Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network.

Chen TC, Lin KT, Chen CH, Lee SA, Lee PY, Liu YW, Kuo YL, Wang FS, Lai JM, Huang CY

Signal transduction pathways in the cell require protein-protein interactions (PPIs) to respond to environmental cues. Diverse experimental techniques for detecting PPIs have been developed. However, the huge amount of PPI data accumulated from various sources poses a challenge with respect to data reliability. Herein, we collected ∼ 700 primary antibodies and employed a highly sensitive and specific technique, an in ... [more]

J. Proteome Res. Dec. 05, 2014; 13(12);5339-46 [Pubmed: 25241761]

Throughput

  • High Throughput

Additional Notes

  • in situ PLA

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RHOA MAPK8
Co-purification
Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Low-BioGRID
244322

Curated By

  • BioGRID