BAIT

PARK2

AR-JP, LPRS2, PDJ, PRKN, KB-152G3.1
parkin RBR E3 ubiquitin protein ligase
GO Process (58)
GO Function (22)
GO Component (12)

Gene Ontology Biological Process

Homo sapiens
PREY

DLG1

DLGH1, SAP-97, SAP97, dJ1061C18.1.1, hdlg
discs, large homolog 1 (Drosophila)
GO Process (17)
GO Function (11)
GO Component (17)
Homo sapiens

Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network.

Chen TC, Lin KT, Chen CH, Lee SA, Lee PY, Liu YW, Kuo YL, Wang FS, Lai JM, Huang CY

Signal transduction pathways in the cell require protein-protein interactions (PPIs) to respond to environmental cues. Diverse experimental techniques for detecting PPIs have been developed. However, the huge amount of PPI data accumulated from various sources poses a challenge with respect to data reliability. Herein, we collected ∼ 700 primary antibodies and employed a highly sensitive and specific technique, an in ... [more]

J. Proteome Res. Dec. 05, 2014; 13(12);5339-46 [Pubmed: 25241761]

Throughput

  • High Throughput

Additional Notes

  • in situ PLA

Curated By

  • BioGRID