BAIT
CACNA1A
BccA1, Cav2.1, rbA-1
calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
GO Process (50)
GO Function (5)
GO Component (11)
Gene Ontology Biological Process
- adult walking behavior [ISO]
- behavioral response to pain [ISO]
- calcium ion import [IDA, IMP]
- calcium ion transmembrane transport [ISO]
- calcium ion transport [IDA, IMP, ISO]
- calcium ion-dependent exocytosis [ISO]
- calcium ion-dependent exocytosis of neurotransmitter [ISO]
- cell death [ISO]
- cell growth [ISO]
- cellular chloride ion homeostasis [ISO]
- central nervous system neuron differentiation [ISO]
- cerebellar Purkinje cell differentiation [ISO]
- cerebellar Purkinje cell layer development [ISO]
- cerebellar molecular layer development [ISO]
- cerebellum maturation [ISO]
- dendrite morphogenesis [ISO]
- gamma-aminobutyric acid secretion [ISO]
- gamma-aminobutyric acid signaling pathway [ISO]
- glucose metabolic process [ISO]
- hormone metabolic process [ISO]
- membrane depolarization [ISO]
- membrane depolarization during action potential [IBA]
- musculoskeletal movement, spinal reflex action [ISO]
- negative regulation of hormone biosynthetic process [ISO]
- negative regulation of neuron apoptotic process [ISO]
- neurological system process [ISO]
- neuromuscular process [ISO]
- neuromuscular process controlling balance [ISO]
- neuromuscular synaptic transmission [ISO]
- neuron-neuron synaptic transmission [ISO]
- neurotransmitter metabolic process [ISO]
- positive regulation of cytosolic calcium ion concentration [ISO, ISS]
- receptor clustering [ISO]
- regulation of acetylcholine secretion, neurotransmission [ISO]
- regulation of axonogenesis [ISO]
- regulation of calcium ion-dependent exocytosis [ISO]
- regulation of ion transmembrane transport [ISO]
- regulation of membrane potential [ISO]
- response to pain [ISO]
- rhythmic synaptic transmission [ISO]
- sensory perception of pain [IMP]
- spinal cord motor neuron differentiation [ISO]
- sulfur amino acid metabolic process [ISO]
- synapse assembly [ISO]
- synaptic transmission [IMP, ISO]
- synaptic transmission, GABAergic [ISO]
- synaptic transmission, glutamatergic [ISO]
- thyroid hormone metabolic process [ISO]
- transmission of nerve impulse [ISO]
- vestibular nucleus development [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
MAP1B
Mtap1b
microtubule-associated protein 1B
GO Process (30)
GO Function (5)
GO Component (16)
Gene Ontology Biological Process
- axon extension [ISO]
- axonogenesis [ISO]
- cellular process [ISO]
- cellular response to growth factor stimulus [IEP]
- cellular response to peptide hormone stimulus [IEP]
- dendrite development [ISO]
- developmental maturation [IEP]
- establishment of monopolar cell polarity [ISO]
- induction of synaptic plasticity by chemical substance [IEP]
- microtubule bundle formation [IDA, ISO]
- mitochondrion transport along microtubule [ISO]
- negative regulation of intracellular transport [ISO]
- negative regulation of microtubule depolymerization [IDA]
- nervous system development [IEP]
- neuron development [IEP]
- peripheral nervous system axon regeneration [IEP]
- positive regulation of axon extension [IMP, ISO]
- positive regulation of microtubule polymerization [IDA]
- positive regulation of neuron differentiation [IMP]
- response to axon injury [IEP]
- response to carbohydrate [IEP]
- response to drug [IEP]
- response to estradiol [IEP]
- response to inorganic substance [IEP]
- response to insecticide [IEP]
- response to mechanical stimulus [IEP]
- response to nutrient levels [IEP]
- response to vitamin A [IEP]
- response to wounding [IEP]
- synapse assembly [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- axon [IDA]
- cell [ISO]
- cytoplasm [ISO]
- cytoskeleton [IDA]
- cytosol [ISO]
- dendrite [IDA]
- growth cone [IDA]
- intracellular [ISO]
- microtubule associated complex [IDA]
- neuronal cell body [IDA]
- neuronal postsynaptic density [ISO]
- perikaryon [IDA]
- perinuclear region of cytoplasm [IDA]
- photoreceptor outer segment [ISO]
- plasma membrane [IDA, ISO]
- postsynaptic density [IDA, ISO]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The MAP1B-LC1/UBE2L3 complex catalyzes degradation of cell surface CaV2.2 channels.
We reported recently a new mechanism by which the neuronal N-type Ca(2+) (CaV2.2) channel expression may be regulated by ubiquitination. This mechanism involves the interaction between the channel and the light chain (LC1) of the microtubule associated protein B (MAP1B). We also showed that MAP1B-LC1 could interact with the ubiquitin-conjugating E2 enzyme UBE2L3 and that the ubiquitination/degradation mechanism triggered by ... [more]
Channels (Austin) Dec. 09, 2014; 8(5);452-7 [Pubmed: 25483588]
Throughput
- Low Throughput
Additional Notes
- Figure 3
Curated By
- BioGRID