POU5F1
Gene Ontology Biological Process
- BMP signaling pathway involved in heart induction [ISO]
- blastocyst growth [IGI]
- cardiac cell fate determination [ISO]
- cell fate commitment [IMP]
- cell fate commitment involved in formation of primary germ layer [ISO]
- ectodermal cell fate commitment [IDA]
- endodermal cell fate commitment [IDA]
- endodermal cell fate specification [ISO]
- germ-line stem cell maintenance [IMP]
- mRNA transcription from RNA polymerase II promoter [IDA]
- mesodermal cell fate commitment [IDA]
- negative regulation of calcium ion-dependent exocytosis [IDA]
- negative regulation of cell differentiation [IGI]
- negative regulation of gene silencing by miRNA [ISO]
- negative regulation of protein kinase B signaling [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of SMAD protein import into nucleus [ISO]
- positive regulation of catenin import into nucleus [ISO]
- positive regulation of protein kinase B signaling [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of gene expression [ISO]
- regulation of heart induction by regulation of canonical Wnt signaling pathway [ISO]
- regulation of methylation-dependent chromatin silencing [ISO]
- regulation of transcription, DNA-templated [IDA, ISO]
- response to organic substance [IDA]
- response to retinoic acid [IDA]
- somatic stem cell maintenance [ISO]
- stem cell differentiation [IDA]
- stem cell maintenance [IMP]
- transcription from RNA polymerase II promoter [IDA, ISO]
- trophectodermal cell differentiation [IGI]
Gene Ontology Molecular Function- DNA binding [IDA, IGI, ISO]
- HMG box domain binding [ISO]
- POU domain binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [ISO]
- chromatin binding [IDA]
- miRNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI, ISO]
- transcription regulatory region DNA binding [IDA, ISO]
- ubiquitin protein ligase binding [IPI, ISO]
- DNA binding [IDA, IGI, ISO]
- HMG box domain binding [ISO]
- POU domain binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [ISO]
- chromatin binding [IDA]
- miRNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI, ISO]
- transcription regulatory region DNA binding [IDA, ISO]
- ubiquitin protein ligase binding [IPI, ISO]
OGT
Gene Ontology Biological Process
- apoptotic process [ISO]
- cellular response to glucose stimulus [ISO]
- cellular response to lipopolysaccharide [ISO]
- cellular response to retinoic acid [ISO]
- circadian regulation of gene expression [IMP]
- glucosamine metabolic process [ISO]
- histone H3-K4 trimethylation [ISO]
- histone H4-K16 acetylation [ISO]
- histone H4-K5 acetylation [ISO]
- histone H4-K8 acetylation [ISO]
- intracellular distribution of mitochondria [ISO]
- negative regulation of cell death [ISO]
- negative regulation of cellular response to hypoxia [ISO]
- negative regulation of peptidyl-serine phosphorylation [ISO]
- negative regulation of peptidyl-threonine phosphorylation [ISO]
- negative regulation of protein phosphorylation [ISO]
- negative regulation of protein targeting to membrane [ISO]
- negative regulation of protein ubiquitination [IDA]
- phosphatidylinositol-mediated signaling [ISO]
- positive regulation of catalytic activity [ISO]
- positive regulation of cell size [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of granulocyte differentiation [ISO]
- positive regulation of histone H3-K27 methylation [ISO]
- positive regulation of histone H3-K4 methylation [ISO]
- positive regulation of protein localization to nucleus [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of proteolysis [ISO]
- positive regulation of reactive oxygen species biosynthetic process [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- protein O-linked glycosylation [IDA, ISO, ISS, TAS]
- protein heterotrimerization [ISO]
- protein homotrimerization [ISO]
- regulation of Rac protein signal transduction [ISO]
- regulation of gluconeogenesis involved in cellular glucose homeostasis [IMP]
- regulation of glycolytic process [ISO]
- regulation of insulin receptor signaling pathway [ISO]
- response to insulin [ISO]
Gene Ontology Molecular Function- N-acetyltransferase activity [TAS]
- catalytic activity [ISS]
- enzyme activator activity [ISO]
- histone acetyltransferase activity (H4-K16 specific) [ISO]
- histone acetyltransferase activity (H4-K5 specific) [ISO]
- histone acetyltransferase activity (H4-K8 specific) [ISO]
- monosaccharide binding [ISO]
- peptide binding [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO]
- protein N-acetylglucosaminyltransferase activity [ISO]
- protein O-GlcNAc transferase activity [IDA, IMP, ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- transcription factor binding [ISO]
- N-acetyltransferase activity [TAS]
- catalytic activity [ISS]
- enzyme activator activity [ISO]
- histone acetyltransferase activity (H4-K16 specific) [ISO]
- histone acetyltransferase activity (H4-K5 specific) [ISO]
- histone acetyltransferase activity (H4-K8 specific) [ISO]
- monosaccharide binding [ISO]
- peptide binding [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO]
- protein N-acetylglucosaminyltransferase activity [ISO]
- protein O-GlcNAc transferase activity [IDA, IMP, ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- transcription factor binding [ISO]
Gene Ontology Cellular Component
- MLL5-L complex [ISO]
- cytoplasm [ISO, TAS]
- cytosol [ISO]
- euchromatin [ISO]
- histone acetyltransferase complex [ISO]
- intracellular [TAS]
- microtubule organizing center [ISO]
- mitochondrion [ISO]
- neuron projection [ISO]
- neuronal cell body [ISO]
- nucleoplasm [ISO]
- nucleus [ISO, TAS]
- plasma membrane [ISO]
- zymogen granule [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
O-GlcNAc regulates pluripotency and reprogramming by directly acting on core components of the pluripotency network.
O-linked-N-acetylglucosamine (O-GlcNAc) has emerged as a critical regulator of diverse cellular processes, but its role in embryonic stem cells (ESCs) and pluripotency has not been investigated. Here we show that O-GlcNAcylation directly regulates core components of the pluripotency network. Blocking O-GlcNAcylation disrupts ESC self-renewal and reprogramming of somatic cells to induced pluripotent stem cells. The core reprogramming factors Oct4 and ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| POU5F1 OGT | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 674379 | |
| POU5F1 OGT | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 471807 | |
| POU5F1 OGT | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| OGT POU5F1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID