BAIT

EGFR

ERBB, ERBB1, HER1, NISBD2, PIG61, mENA
epidermal growth factor receptor
GO Process (42)
GO Function (15)
GO Component (13)

Gene Ontology Biological Process

Homo sapiens

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells.

Petschnigg J, Groisman B, Kotlyar M, Taipale M, Zheng Y, Kurat CF, Sayad A, Sierra JR, Mattiazzi Usaj M, Snider J, Nachman A, Krykbaeva I, Tsao MS, Moffat J, Pawson T, Lindquist S, Jurisica I, Stagljar I

Cell signaling, one of key processes in both normal cellular function and disease, is coordinated by numerous interactions between membrane proteins that change in response to stimuli. We present a split ubiquitin-based method for detection of integral membrane protein-protein interactions (PPIs) in human cells, termed mammalian-membrane two-hybrid assay (MaMTH). We show that this technology detects stimulus (hormone or agonist)-dependent and ... [more]

Nat. Methods May. 01, 2014; 11(5);585-92 [Pubmed: 24658140]

Throughput

  • High Throughput

Additional Notes

  • Mammalian-membrane two-hybrid assay (MaMTH)
  • Split ubiquitin-based method

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EGFR MAPK8IP2
Protein-peptide
Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

High-BioGRID
833541
EGFR MAPK8IP2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID