EGFR
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- MAPK cascade [NAS]
- activation of phospholipase A2 activity by calcium-mediated signaling [TAS]
- activation of phospholipase C activity [TAS]
- axon guidance [TAS]
- cell proliferation [IDA]
- cell surface receptor signaling pathway [IDA]
- cellular response to epidermal growth factor stimulus [ISS]
- cellular response to estradiol stimulus [IDA]
- epidermal growth factor receptor signaling pathway [IDA, TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- learning or memory [ISS]
- negative regulation of apoptotic process [IMP]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of protein catabolic process [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- ossification [NAS]
- peptidyl-tyrosine phosphorylation [IDA, IMP, TAS]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of DNA repair [IDA]
- positive regulation of DNA replication [IDA]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of MAP kinase activity [IDA]
- positive regulation of catenin import into nucleus [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IDA]
- positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle [IDA]
- positive regulation of epithelial cell proliferation [IDA]
- positive regulation of nitric oxide biosynthetic process [IDA]
- positive regulation of phosphorylation [IDA]
- positive regulation of protein kinase B signaling [IMP]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein autophosphorylation [IMP]
- protein insertion into membrane [TAS]
- regulation of nitric-oxide synthase activity [IDA]
- regulation of peptidyl-tyrosine phosphorylation [IMP]
- response to UV-A [IDA]
- response to stress [NAS]
- signal transduction [IDA, TAS]
- single organismal cell-cell adhesion [IMP]
Gene Ontology Molecular Function- MAP kinase kinase kinase activity [NAS]
- actin filament binding [IDA]
- chromatin binding [IDA]
- double-stranded DNA binding [NAS]
- enzyme binding [IPI]
- epidermal growth factor-activated receptor activity [IDA, NAS]
- identical protein binding [IPI]
- nitric-oxide synthase regulator activity [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- protein phosphatase binding [IPI]
- protein tyrosine kinase activity [IDA, IMP, TAS]
- transmembrane receptor protein tyrosine kinase activity [TAS]
- transmembrane signaling receptor activity [IDA]
- ubiquitin protein ligase binding [IPI]
- MAP kinase kinase kinase activity [NAS]
- actin filament binding [IDA]
- chromatin binding [IDA]
- double-stranded DNA binding [NAS]
- enzyme binding [IPI]
- epidermal growth factor-activated receptor activity [IDA, NAS]
- identical protein binding [IPI]
- nitric-oxide synthase regulator activity [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- protein phosphatase binding [IPI]
- protein tyrosine kinase activity [IDA, IMP, TAS]
- transmembrane receptor protein tyrosine kinase activity [TAS]
- transmembrane signaling receptor activity [IDA]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
ARRB1
Gene Ontology Biological Process
- G-protein coupled receptor internalization [IMP]
- Notch signaling pathway [TAS]
- blood coagulation [TAS]
- membrane organization [TAS]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of interleukin-6 production [IDA]
- negative regulation of interleukin-8 production [IDA]
- negative regulation of protein ubiquitination [IDA]
- platelet activation [TAS]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of GTPase activity [IMP]
- positive regulation of Rho protein signal transduction [IMP]
- positive regulation of histone H4 acetylation [IMP]
- positive regulation of histone acetylation [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of receptor internalization [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- post-Golgi vesicle-mediated transport [TAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein ubiquitination [IMP]
- stress fiber assembly [IMP]
- transcription from RNA polymerase II promoter [IMP]
Gene Ontology Molecular Function- GTPase activator activity [IMP]
- angiotensin receptor binding [IPI]
- enzyme inhibitor activity [TAS]
- histone acetyltransferase activity [IDA]
- insulin-like growth factor receptor binding [IPI]
- protein binding [IPI]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IMP]
- ubiquitin protein ligase binding [IPI]
- GTPase activator activity [IMP]
- angiotensin receptor binding [IPI]
- enzyme inhibitor activity [TAS]
- histone acetyltransferase activity [IDA]
- insulin-like growth factor receptor binding [IPI]
- protein binding [IPI]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IMP]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
Affinity Capture-Luminescence
An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.
Publication
The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells.
Cell signaling, one of key processes in both normal cellular function and disease, is coordinated by numerous interactions between membrane proteins that change in response to stimuli. We present a split ubiquitin-based method for detection of integral membrane protein-protein interactions (PPIs) in human cells, termed mammalian-membrane two-hybrid assay (MaMTH). We show that this technology detects stimulus (hormone or agonist)-dependent and ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EGFR ARRB1 | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | High | - | BioGRID | 1505963 | |
| EGFR ARRB1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID