An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.

Li X, Wang W, Wang J, Malovannaya A, Xi Y, Li W, Guerra R, Hawke DH, Qin J, Chen J

The current knowledge on how transcription factors (TFs), the ultimate targets and executors of cellular signalling pathways, are regulated by protein-protein interactions remains limited. Here, we performed proteomics analyses of soluble and chromatin-associated complexes of 56 TFs, including the targets of many signalling pathways involved in development and cancer, and 37 members of the Forkhead box (FOX) TF family. Using ... [more]

Mol. Syst. Biol. Jan. 01, 2015; 11(1);775 [Pubmed: 25609649]

Throughput

High Throughput

Additional Notes

High-confidence interactions

Curated By

BioGRID

Interaction Summary

ETS1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [ISS]protein binding [IPI]sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]sequence-specific DNA binding transcription factor activity [IDA]transcription factor binding [NAS]

Gene Ontology Cellular Component

ATP2B1