An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.

Li X, Wang W, Wang J, Malovannaya A, Xi Y, Li W, Guerra R, Hawke DH, Qin J, Chen J

The current knowledge on how transcription factors (TFs), the ultimate targets and executors of cellular signalling pathways, are regulated by protein-protein interactions remains limited. Here, we performed proteomics analyses of soluble and chromatin-associated complexes of 56 TFs, including the targets of many signalling pathways involved in development and cancer, and 37 members of the Forkhead box (FOX) TF family. Using ... [more]

Mol. Syst. Biol. Jan. 01, 2015; 11(1);775 [Pubmed: 25609649]

Throughput

High Throughput

Additional Notes

High-confidence interactions

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TEAD2 TRIM3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2699832

Curated By

BioGRID

Interaction Summary

TEAD2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]sequence-specific DNA binding transcription factor activity [IDA, NAS]

Gene Ontology Cellular Component

TRIM3

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein C-terminus binding [TAS]protein binding [IPI]