BAIT

USP7

fc48f04, wu:fc48f04
ubiquitin specific peptidase 7 (herpes virus-associated)
GO Process (0)
GO Function (0)
GO Component (0)
Danio rerio
PREY

GLI3

im:7142603
GLI family zinc finger 3
GO Process (3)
GO Function (1)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Danio rerio

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Deubiquitination of Ci/Gli by Usp7/HAUSP Regulates Hedgehog Signaling.

Zhou Z, Yao X, Li S, Xiong Y, Dong X, Zhao Y, Jiang J, Zhang Q

Hedgehog (Hh) signaling plays essential roles in animal development and tissue homeostasis, and its misregulation causes congenital diseases and cancers. Regulation of the ubiquitin/proteasome-mediated proteolysis of Ci/Gli transcription factors is central to Hh signaling, but whether deubiquitinase is involved in this process remains unknown. Here, we show that Hh stimulates the binding of a ubiquitin-specific protease Usp7 to Ci, which ... [more]

Dev. Cell Jul. 06, 2015; 34(1);58-72 [Pubmed: 26120032]

Throughput

  • Low Throughput

Curated By

  • BioGRID