BAIT
HECW1
NEDL1
HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
GO Process (1)
GO Function (1)
GO Component (0)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
UBE2D2
E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B
ubiquitin-conjugating enzyme E2D 2
GO Process (13)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- MyD88-independent toll-like receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- cellular protein modification process [TAS]
- cellular response to hypoxia [TAS]
- innate immune response [TAS]
- protein K48-linked ubiquitination [IDA]
- protein polyubiquitination [IDA]
- protein ubiquitination [IDA]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Assembly and structure of Lys33-linked polyubiquitin reveals distinct conformations.
Ubiquitylation regulates a multitude of biological processes and this versatility stems from the ability of ubiquitin (Ub) to form topologically different polymers of eight different linkage types. Whereas some linkages have been studied in detail, other linkage types including Lys33-linked polyUb are poorly understood. In the present study, we identify an enzymatic system for the large-scale assembly of Lys33 chains by ... [more]
Biochem. J. Apr. 15, 2015; 467(2);345-52 [Pubmed: 25723849]
Throughput
- Low Throughput
Additional Notes
- Polyubiquitin chain assembly in vitro.
- The separate reactions contained E1, different E2s and HECT E3s.
Curated By
- BioGRID