Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Mutant p53 protects ETS2 from non-canonical COP1/DET1 dependent degradation.

Carrero ZI, Kollareddy M, Chauhan KM, Ramakrishnan G, Martinez LA

Mutations in the tumor suppressor gene TP53 contribute to the development of approximately half of all human cancers. One mechanism by which mutant p53 (mtp53) acts is through interaction with other transcription factors, which can either enhance or repress the transcription of their target genes. Mtp53 preferentially interacts with the erythroblastosis virus E26 oncogene homologue 2 (ETS2), an ETS transcription ... [more]

Oncotarget Feb. 09, 2016; 0(0); [Pubmed: 26871468]

Throughput

  • Low Throughput

Curated By

  • BioGRID