AXIN2
Gene Ontology Biological Process
- Wnt signaling pathway involved in somitogenesis [IBA]
- cellular protein localization [IDA]
- cellular response to organic cyclic compound [IBA]
- dorsal/ventral axis specification [IBA]
- intramembranous ossification [IBA]
- mRNA stabilization [IMP]
- maintenance of DNA repeat elements [IMP]
- negative regulation of canonical Wnt signaling pathway [IDA, IMP]
- negative regulation of catenin import into nucleus [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of osteoblast differentiation [IBA]
- odontogenesis [IMP]
- positive regulation of GTPase activity [IBA]
- positive regulation of cell death [IMP]
- positive regulation of epithelial to mesenchymal transition [IMP]
- positive regulation of protein phosphorylation [IMP]
- regulation of centromeric sister chromatid cohesion [IMP]
- regulation of mismatch repair [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SMAD7
Gene Ontology Biological Process
- BMP signaling pathway [TAS]
- adherens junction assembly [IMP]
- artery morphogenesis [ISS]
- cellular protein complex localization [IDA]
- cellular response to transforming growth factor beta stimulus [IMP]
- gene expression [TAS]
- negative regulation of BMP signaling pathway [IDA]
- negative regulation of cell migration [TAS]
- negative regulation of epithelial to mesenchymal transition [IC, TAS]
- negative regulation of pathway-restricted SMAD protein phosphorylation [IDA, TAS]
- negative regulation of peptidyl-serine phosphorylation [IDA]
- negative regulation of peptidyl-threonine phosphorylation [IDA]
- negative regulation of protein ubiquitination [IDA]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of transcription by competitive promoter binding [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [IDA, TAS]
- negative regulation of ubiquitin-protein transferase activity [IDA]
- pathway-restricted SMAD protein phosphorylation [ISS]
- positive regulation of cell-cell adhesion [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein ubiquitination [IDA]
- positive regulation of transcription from RNA polymerase II promoter [TAS]
- protein stabilization [IDA]
- regulation of activin receptor signaling pathway [IDA]
- regulation of cardiac muscle contraction [ISS]
- regulation of transforming growth factor beta receptor signaling pathway [IC]
- regulation of ventricular cardiac muscle cell membrane depolarization [IC]
- response to laminar fluid shear stress [IEP]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- ventricular cardiac muscle tissue morphogenesis [ISS]
- ventricular septum morphogenesis [ISS]
Gene Ontology Molecular Function- I-SMAD binding [IPI]
- activin binding [IPI]
- beta-catenin binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA]
- type I transforming growth factor beta receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- I-SMAD binding [IPI]
- activin binding [IPI]
- beta-catenin binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA]
- type I transforming growth factor beta receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Nuclear receptor NR4A1 promotes breast cancer invasion and metastasis by activating TGF-β signalling.
In advanced cancers, the TGF-β pathway acts as an oncogenic factor and is considered to be a therapeutic target. Here using a genome-wide cDNA screen, we identify nuclear receptor NR4A1 as a strong activator of TGF-β signalling. NR4A1 promotes TGF-β/SMAD signalling by facilitating AXIN2-RNF12/ARKADIA-induced SMAD7 degradation. NR4A1 interacts with SMAD7 and AXIN2, and potently and directly induces AXIN2 expression. Whereas ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure S4
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SMAD7 AXIN2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID