BAIT

TMUB1

C7orf21, DULP, SB144
transmembrane and ubiquitin-like domain containing 1
UPS Project
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens
PREY

RANGAP1

Fug1, RANGAP, SD, RP4-756G23.7
Ran GTPase activating protein 1
GO Process (3)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Different functions of HOPS isoforms in the cell: HOPS shuttling isoform is determined by RIP cleavage system.

Castelli M, Piobbico D, Bartoli D, Pieroni S, Brunacci C, Bellet MM, Chiacchiaretta M, Della Fazia MA, Servillo G

Hepatocyte odd protein shuttling (HOPS) moves between nucleus and cytoplasm. HOPS overexpression leads to cell cycle arrest in G 0/G 1, and HOPS knockdown causes centrosome alterations, with subsequent abnormal cell division. Recently, we demonstrated that HOPS acts as a functional bridge in NPM-p19(Arf) interactions. Here we show that HOPS is present in 3 different isoforms that play distinct intracellular ... [more]

Cell Cycle Nov. 19, 2013; 13(2);293-302 [Pubmed: 24240191]

Throughput

  • Low Throughput

Additional Notes

  • Figure 4
  • source of proteins unclear

Curated By

  • BioGRID