ARRB2
Gene Ontology Biological Process
- G-protein coupled receptor internalization [ISO]
- G-protein coupled receptor signaling pathway [IDA]
- adult walking behavior [ISO]
- brain development [IEP]
- cell chemotaxis [ISO]
- desensitization of G-protein coupled receptor protein signaling pathway by arrestin [IMP, ISO, TAS]
- detection of temperature stimulus involved in sensory perception of pain [IMP]
- endocytosis [IDA]
- follicle-stimulating hormone signaling pathway [IDA]
- negative regulation of GTPase activity [IDA]
- negative regulation of NF-kappaB transcription factor activity [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of interleukin-1 beta production [ISO]
- negative regulation of interleukin-12 production [ISO]
- negative regulation of interleukin-6 production [ISO]
- negative regulation of natural killer cell mediated cytotoxicity [ISO]
- negative regulation of protein ubiquitination [IMP, ISO]
- negative regulation of release of cytochrome c from mitochondria [IMP]
- negative regulation of smooth muscle cell apoptotic process [IMP]
- negative regulation of toll-like receptor signaling pathway [ISO]
- negative regulation of tumor necrosis factor production [ISO]
- positive regulation of DNA biosynthetic process [IMP]
- positive regulation of ERK1 and ERK2 cascade [IMP, ISO]
- positive regulation of calcium ion transport [IMP]
- positive regulation of peptidyl-serine phosphorylation [IMP]
- positive regulation of peptidyl-tyrosine phosphorylation [IMP]
- positive regulation of protein kinase B signaling [IMP, ISO]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of protein ubiquitination [ISO]
- positive regulation of receptor internalization [IMP, ISO]
- positive regulation of synaptic transmission, dopaminergic [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein ubiquitination [ISO]
- receptor internalization [ISO]
- regulation of G-protein coupled receptor protein signaling pathway [IDA, ISO]
- regulation of androgen receptor signaling pathway [ISO]
- regulation of cAMP biosynthetic process [TAS]
- regulation of cAMP catabolic process [TAS]
- regulation of protein phosphorylation [ISO]
- transcription from RNA polymerase II promoter [ISO]
- transforming growth factor beta receptor signaling pathway [ISO]
Gene Ontology Molecular Function- 14-3-3 protein binding [IDA]
- D1 dopamine receptor binding [IPI]
- G-protein coupled receptor binding [ISO, TAS]
- alpha-1A adrenergic receptor binding [IPI]
- alpha-1B adrenergic receptor binding [IDA]
- angiotensin receptor binding [ISO]
- clathrin binding [TAS]
- enzyme binding [ISO]
- follicle-stimulating hormone receptor binding [IPI]
- mitogen-activated protein kinase binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA]
- protein complex scaffold [ISO]
- protein domain specific binding [IPI]
- protein kinase B binding [ISO]
- receptor binding [ISO]
- type 1 angiotensin receptor binding [IPI]
- type 2A serotonin receptor binding [IPI]
- ubiquitin protein ligase binding [ISO]
- 14-3-3 protein binding [IDA]
- D1 dopamine receptor binding [IPI]
- G-protein coupled receptor binding [ISO, TAS]
- alpha-1A adrenergic receptor binding [IPI]
- alpha-1B adrenergic receptor binding [IDA]
- angiotensin receptor binding [ISO]
- clathrin binding [TAS]
- enzyme binding [ISO]
- follicle-stimulating hormone receptor binding [IPI]
- mitogen-activated protein kinase binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA]
- protein complex scaffold [ISO]
- protein domain specific binding [IPI]
- protein kinase B binding [ISO]
- receptor binding [ISO]
- type 1 angiotensin receptor binding [IPI]
- type 2A serotonin receptor binding [IPI]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
USP20
Gene Ontology Biological Process
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein K48-linked deubiquitination [IDA]
- protein K63-linked deubiquitination [IDA]
- protein deubiquitination [IDA]
- regulation of G-protein coupled receptor protein signaling pathway [IMP]
- regulation of proteasomal protein catabolic process [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Ubiquitin-specific protease 20 Regulates the Reciprocal Functions of Beta-arrestin2 in Toll-like Receptor 4-promoted NFkappaB Activation.
Toll-like receptor 4 (TLR4) promotes vascular inflammatory disorders such as neointimal hyperplasia and atherosclerosis. TLR4 triggers NFκB signaling through the ubiquitin ligase TRAF6 (Tumor Necrosis Factor Receptor-associated factor 6). TRAF6 activity can be impeded by deubiquitinating enzymes like ubiquitin-specific protease 20 (USP20), which can reverse TRAF6 auto-ubiquitination, and by association with the multi-functional adaptor protein β-arrestin2. Whereas β-arrestin2 effects on ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| USP20 ARRB2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID