CRY1
Gene Ontology Biological Process
- DNA damage induced protein phosphorylation [ISS]
- blue light signaling pathway [NAS]
- circadian regulation of gene expression [ISS]
- entrainment of circadian clock by photoperiod [ISS]
- gluconeogenesis [ISS]
- glucose homeostasis [ISS]
- negative regulation of G-protein coupled receptor protein signaling pathway [ISS]
- negative regulation of circadian rhythm [ISS]
- negative regulation of glucocorticoid receptor signaling pathway [ISS]
- negative regulation of protein ubiquitination [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA, ISS]
- regulation of DNA damage checkpoint [ISS]
- regulation of circadian rhythm [ISS]
- response to glucagon [ISS]
Gene Ontology Molecular Function- DNA (6-4) photolyase activity [IDA]
- DNA binding [TAS]
- blue light photoreceptor activity [NAS]
- core promoter binding [ISS]
- deoxyribodipyrimidine photo-lyase activity [IDA]
- double-stranded DNA binding [IDA]
- nuclear hormone receptor binding [IPI]
- phosphatase binding [IPI]
- protein binding [IPI]
- transcription factor binding transcription factor activity [IDA]
- ubiquitin binding [ISS]
- DNA (6-4) photolyase activity [IDA]
- DNA binding [TAS]
- blue light photoreceptor activity [NAS]
- core promoter binding [ISS]
- deoxyribodipyrimidine photo-lyase activity [IDA]
- double-stranded DNA binding [IDA]
- nuclear hormone receptor binding [IPI]
- phosphatase binding [IPI]
- protein binding [IPI]
- transcription factor binding transcription factor activity [IDA]
- ubiquitin binding [ISS]
ARNTL
Gene Ontology Biological Process
- circadian regulation of gene expression [IDA, ISS]
- circadian rhythm [TAS]
- negative regulation of TOR signaling [ISS]
- negative regulation of fat cell differentiation [ISS]
- negative regulation of glucocorticoid receptor signaling pathway [ISS]
- negative regulation of transcription, DNA-templated [ISS]
- oxidative stress-induced premature senescence [ISS]
- positive regulation of canonical Wnt signaling pathway [ISS]
- positive regulation of circadian rhythm [ISS]
- positive regulation of skeletal muscle cell differentiation [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI]
- positive regulation of transcription, DNA-templated [IDA, IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISS]
- regulation of cell cycle [ISS]
- regulation of cellular senescence [ISS]
- regulation of hair cycle [IMP]
- regulation of insulin secretion [ISS]
- regulation of neurogenesis [ISS]
- regulation of transcription, DNA-templated [ISS]
- regulation of type B pancreatic cell development [ISS]
- response to redox state [IDA]
- spermatogenesis [ISS]
Gene Ontology Molecular Function- DNA binding [IDA, IGI]
- E-box binding [IDA]
- Hsp90 protein binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity [ISS]
- aryl hydrocarbon receptor binding [IPI]
- core promoter binding [ISS]
- protein binding [IPI]
- sequence-specific DNA binding [ISS]
- transcription regulatory region sequence-specific DNA binding [ISS]
- DNA binding [IDA, IGI]
- E-box binding [IDA]
- Hsp90 protein binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity [ISS]
- aryl hydrocarbon receptor binding [IPI]
- core promoter binding [ISS]
- protein binding [IPI]
- sequence-specific DNA binding [ISS]
- transcription regulatory region sequence-specific DNA binding [ISS]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization.
The circadian transcriptional repressors cryptochrome 1 (Cry1) and 2 (Cry2) evolved from photolyases, bacterial light-activated DNA repair enzymes. In this study, we report that while they have lost DNA repair activity, Cry1/2 adapted to protect genomic integrity by responding to DNA damage through posttranslational modification and coordinating the downstream transcriptional response. We demonstrate that genotoxic stress stimulates Cry1 phosphorylation and ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CRY1 ARNTL | Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag. | Low | - | BioGRID | - | |
| CRY1 ARNTL | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CRY1 ARNTL | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | - | |
| ARNTL CRY1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| ARNTL CRY1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID