An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

CDKB1;1 forms a functional complex with CYCA2;3 to suppress endocycle onset.

Boudolf V, Lammens T, Boruc J, Van Leene J, Van Den Daele H, Maes S, Van Isterdael G, Russinova E, Kondorosi E, Witters E, De Jaeger G, Inze D, De Veylder L

The mitosis-to-endocycle transition requires the controlled inactivation of M phase-associated cyclin-dependent kinase (CDK) activity. Previously, the B-type CDKB1;1 was identified as an important negative regulator of endocycle onset. Here, we demonstrate that CDKB1;1 copurifies and associates with the A2-type cyclin CYCA2;3. Coexpression of CYCA2;3 with CDKB1;1 triggered ectopic cell divisions and inhibited endoreduplication. Moreover, the enhanced endoreduplication phenotype observed after ... [more]

Plant Physiol. Jul. 01, 2009; 150(3);1482-93 [Pubmed: 19458112]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CYCA2;3 APX1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Van Leene J (2010)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CYCA2;3

Gene Ontology Biological Process

Gene Ontology Molecular Function

cyclin-dependent protein serine/threonine kinase regulator activity [ISS]protein binding [IPI]

Gene Ontology Cellular Component

APX1