BAIT

CAB39

AA408805, AA960512, C78372, MO25, MO25alpha
calcium binding protein 39
Mus musculus
PREY

STK26

2610018G03Rik, Mst4, RP23-245F8.1
serine/threonine kinase 26
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The kinase MST4 limits inflammatory responses through direct phosphorylation of the adaptor TRAF6.

Jiao S, Zhang Z, Li C, Huang M, Shi Z, Wang Y, Song X, Liu H, Li C, Chen M, Wang W, Zhao Y, Jiang Z, Wang H, Wong CC, Wang C, Zhou Z

Immune responses need to be tightly controlled to avoid excessive inflammation and prevent unwanted host damage. Here we report that germinal center kinase MST4 responded dynamically to bacterial infection and acted as a negative regulator of inflammation. We found that MST4 directly interacted with and phosphorylated the adaptor TRAF6 to prevent its oligomerization and autoubiquitination. Accordingly, MST4 did not inhibit ... [more]

Nat. Immunol. Mar. 01, 2015; 16(3);246-57 [Pubmed: 25642822]

Throughput

  • Low Throughput

Additional Notes

  • Figure S3

Curated By

  • BioGRID