BAIT

NTRK1

Trk
neurotrophic tyrosine kinase, receptor, type 1
GO Process (42)
GO Function (9)
GO Component (13)

Gene Ontology Biological Process

Rattus norvegicus
PREY

TRAF6

TNF receptor-associated factor 6, E3 ubiquitin protein ligase
GO Process (44)
GO Function (11)
GO Component (12)

Gene Ontology Biological Process

Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Amyloid β-abrogated TrkA ubiquitination in PC12 cells analogous to Alzheimer's disease.

Zheng C, Geetha T, Gearing M, Babu JR

Amyloid beta (Aβ) protein is the primary proteinaceous deposit found in the brains of patients with Alzheimer's disease (AD). Evidence suggests that Aβ plays a central role in the development of AD pathology. Here, we show in PC12 cells, Aβ impairs tropomyosin receptor kinase A (TrkA) ubiquitination, phosphorylation, and its association with p75(NTR), p62, and TRAF6 induced by nerve growth ... [more]

J. Neurochem. Jun. 01, 2015; 133(6);919-25 [Pubmed: 25708205]

Throughput

  • Low Throughput

Additional Notes

  • Figure 2

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NTRK1 TRAF6
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID